Products/Services Used | Details | Operation |
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Monoclonal Antibody Services> | The following primary antibodies were used for confocal microscopy : LC3 (mouse monoclonal, MBL, M152-3), c-Myc (mouse monoclonal, MBL, M192-3), PbHSP70 (2E638), PbUIS429 (goat polyclonal, SicGen, AB0042-200), LAMP1 (rabbit polyclonal, Sigma, L1418) and PbMSP129 (rabbit polyclonal, GenScript, custom- made). | Get A Quote |
The causative agent of malaria, Plasmodium, replicates inside a membrane-bound parasitophorous vacuole (PV), which shields this intracellular parasite from the cytosol of the host cell 1 . One common threat for intracellular pathogens is the homeostatic process of autophagy, through which cells capture unwanted intracellular material for lysosomal degradation 2 . During the liver stage of a malaria infection, Plasmodium parasites are targeted by the autophagy machinery of the host cell, and the PV membrane (PVM) becomes decorated with several autophagy markers, including LC3 (microtubule-associated protein 1 light chain 3) 3,4 . Here we show that Plasmodium berghei parasites infecting hepatic cells rely on the ... More