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A time course of orchestrated endophilin action in sensing, bending, and stabilizing curved membranes.

Mol Biol Cell. 2016; 
Poudel KR, Dong Y, Yu H, Su A, Ho T, Liu Y, Schulten K, Bai J.
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Monoclonal Antibody Services EndoBAR was detected using a mouse monoclonal antibody against endophilin A1 (Abcam 55702) and a horseradish peroxidase conjugated goat anti-mouse secondary antibody (Genscript), and was visualized using enhanced chemiluminescence. Get A Quote

摘要

Numerous proteins act in concert to sculpt membrane compartments for cell signaling and metabolism. These proteins may act as curvature sensors, membrane benders, and scaffolding molecules. Here we show that endophilin, a critical protein for rapid endocytosis, quickly transforms from a curvature sensor into an active bender upon membrane association. We find that local membrane deformation does not occur until endophilin inserts its amphipathic helices into lipid bilayers, supporting an active bending mechanism through wedging. Our time-course studies show that endophilin continues to drive membrane changes on a seconds-to-minutes time scale, indicating that the duration of endocytosis events constrains the mo... More

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