至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Grouper (Epinephelus coioides) Mpeg1s: Molecular identification, expression analysis, and antimicrobial activity.

Fish Shellfish Immunol. 2019; 
Ni LY, Han Q, Chen HP, Luo XC, Li AX, Dan XM, Li YW.
Products/Services Used Details Operation
Biochemicals … OD) reached 0.6, 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG) was added to induce expression of the recombinant protein for 4 h at 37 °C. The recombinant proteins rEcMpeg1a and rEcMpeg1b were purified using Ni Resin FF (GenScript, Nanjing, China) according to the … Get A Quote

摘要

Macrophage expressed gene 1 (Mpeg1) is a molecule that can form pores and destroy the cell membrane of invading pathogens. In this study, we identified two Mpeg1 isoforms from the orange-spotted grouper (Epinephelus coioides) and named them EcMpeg1a and EcMpeg1b. Predicted proteins of the two EcMpeg1s contained a signal peptide, a conserved membrane attack complex/perforin (MACPF) domain, a transmembrane segment, and an intracellular region. Sequence alignment demonstrated that two EcMpeg1 proteins share a high sequence identity with that of other teleosts. Tissue distribution analysis showed that EcMpeg1s were expressed in all tissues tested in healthy grouper, with the highest expression in the head kidney an... More

关键词

Antibacterial activity; Antiparasitic activity; Cryptocaryon irritans; Epinephelus coioides; Macrophage expressed gene 1