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Biochemicals> | … OD) reached 0.6, 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG) was added to induce expression of the recombinant protein for 4 h at 37 °C. The recombinant proteins rEcMpeg1a and rEcMpeg1b were purified using Ni Resin FF (GenScript, Nanjing, China) according to the … | Get A Quote |
Macrophage expressed gene 1 (Mpeg1) is a molecule that can form pores and destroy the cell membrane of invading pathogens. In this study, we identified two Mpeg1 isoforms from the orange-spotted grouper (Epinephelus coioides) and named them EcMpeg1a and EcMpeg1b. Predicted proteins of the two EcMpeg1s contained a signal peptide, a conserved membrane attack complex/perforin (MACPF) domain, a transmembrane segment, and an intracellular region. Sequence alignment demonstrated that two EcMpeg1 proteins share a high sequence identity with that of other teleosts. Tissue distribution analysis showed that EcMpeg1s were expressed in all tissues tested in healthy grouper, with the highest expression in the head kidney an... More