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Identification of a Type-Specific Epitope in the ORF2 Protein of Duck Astrovirus Type 1.

Animals (Basel). 2019; 
Lan J,, Zhang R,, Li P, Chen J,, Xie Z,, Jiang S,.
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ORF cDNA Clones/MolecularCloud … thiogalactoside (IPTG). All the GST-fusion ORF2 proteins were purified using glutathione resins (Genscript, Piscataway, NJ, USA) and subsequently analyzed by dot blot assay according to the previous study [29]. Briefly, approximately … Get A Quote

摘要

Duck astrovirus type 1 (DAstV-1) infection constitutes a cause of viral hepatitis in ducklings and little is known about the B-cell epitope of DAstV-1. In this study, a monoclonal antibody (mAb) 3D2 against open reading frame 2 (ORF2) protein of DAstV-1 was used to identify the possible epitope in the four serotypes of DAstV. The mAb 3D2 showed no neutralization activity to DAstV-1, and reacted with the conserved linear B-cell epitopes of 454STTESA459 in DAstV-1 ORF2 protein. Sequence analysis, dot blot assay, and cross-reactivity test indicated that the epitope peptide was highly conserved in DAstV-1 sequence and mAb 3D2 had no cross-reactivity with other DAstV serotypes. To the best of our knowledge, this is ... More

关键词

B-cell epitope; DAstV-1; ORF2 protein; mAb