| Products/Services Used | Details | Operation |
|---|---|---|
| Antibody Modification and Purification Services | … The mouse monoclonal antibody isotyping kit was purchased from Southern Biotech (Birmingham, AL, USA). Horseradish peroxidase (HRP) and goat anti-mouse IgG (H+L) HRP were purchased from GenScript (Nanjing, China). 4.2. Production of mAbs Against Salmonella … | Get A Quote |
Salmonella, a common foodborne pathogen, causes many cases of foodborne illness and poses a threat to public health worldwide. Immunological detection systems can be combined with nanoparticles to develop sensitive and portable detection technologies for timely screening of Salmonella infections. Here, we developed an antibody-probe-based immuno-N-hydroxysuccinimide (NHS) bead (AIB) system to detect Salmonella. After adding the antibody probe, Salmonella accumulated in the samples on the surfaces of the immuno-NHS beads (INBs), forming a sandwich structure (INB-Salmonella-probes). We demonstrated the utility of our AIB diagnostic system for detecting Salmonella in water, milk, and eggs, with a sensitivity of 9 ... More
