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Instrument-Free and Visual Detection of Salmonella Based on Magnetic Nanoparticles and an Antibody Probe Immunosensor.

Int J Mol Sci. 2019-09; 
Zhang L,,, Du X, Chen Z, Chen C, Gong N, Song Y, Song Y, Han Q, Xia X, Luo H,, Zhang J.
Products/Services Used Details Operation
Antibody Modification and Purification Services … The mouse monoclonal antibody isotyping kit was purchased from Southern Biotech (Birmingham, AL, USA). Horseradish peroxidase (HRP) and goat anti-mouse IgG (H+L) HRP were purchased from GenScript (Nanjing, China). 4.2. Production of mAbs Against Salmonella … Get A Quote

摘要

Salmonella, a common foodborne pathogen, causes many cases of foodborne illness and poses a threat to public health worldwide. Immunological detection systems can be combined with nanoparticles to develop sensitive and portable detection technologies for timely screening of Salmonella infections. Here, we developed an antibody-probe-based immuno-N-hydroxysuccinimide (NHS) bead (AIB) system to detect Salmonella. After adding the antibody probe, Salmonella accumulated in the samples on the surfaces of the immuno-NHS beads (INBs), forming a sandwich structure (INB-Salmonella-probes). We demonstrated the utility of our AIB diagnostic system for detecting Salmonella in water, milk, and eggs, with a sensitivity of 9 ... More

关键词

HRP-probe; Salmonella; immunoassay; magnetic nanoparticles; monoclonal antibody