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Inhibition of mTOR Alleviates Early Brain Injury After Subarachnoid Hemorrhage Via Relieving Excessive Mitochondrial Fission.

Cell Mol Neurobiol. 2019; 
Li Y,, Wu P, Dai J, Zhang T, Bihl J, Wang C, Liu Y, Shi H.
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Nucleic Acid Purification & Analysis … Briefly, the samples (50 μg) were loaded onto PAGE gels (Genscript, Piscataway Township, NJ, USA), and following electrophoresis, were transferred to nitrocellulose membranes (Life Technologies) and blocked with 5% nonfat milk for 1 h at 37 °C. The membranes were then … Get A Quote

摘要

The mammalian target of rapamycin (mTOR) was reported to regulate cell autophagy and outcomes of several neurological diseases. Mitochondria, which serve as critical organelles in neurons. are also involved in the pathology of neurological diseases. However, the role of mTOR in mitochondrial morphology has not been clarified especially in subarachnoid hemorrhage (SAH). In this study, we established SAH models both in vivo and in vitro. Rapamycin and 3-methyl adenine (3-MA) were then administered to alter mTOR activity. Post-SAH assessment included SAH grading, neurological evaluation, blood-brain barrier (BBB) permeability, brain water content, mitochondrial membrane potential (MMP), mitochondrial morphology, A... More

关键词

Early brain injury; MTOR; Mitochondrial fission; Subarachnoid hemorrhage