| Products/Services Used | Details | Operation |
|---|---|---|
| DNA Sequencing | … All the constructed plasmids were sequenced and confirmed by GenScript (Nanjing, Jiangsu, China) … The comparison of the amino acid sequences of β2-ARs from cattle, sheep, pig, hamster, human, monkey, dog, and cat demonstrates more than 90% identities with each other … | Get A Quote |
β-Adrenergic agonists (β-agonists) are illegally used in animal husbandry, threatening the health of consumers. To realize multianalyte detection of β-agonists, a β2-adrenergic receptor (β2-AR) was cloned from Syrian hamster lung and heterogeneously expressed by Spodoptera frugiperda (Sf9) cells. The recombinant β2-AR was purified from intracellular soluble proteins of infected Sf9 cells, and was utilized to establish an enzyme-linked-receptor assay (ELRA) to detect a group of β-agonists simultaneously. This assay was based on direct competitive inhibition of binding of horseradish peroxidase-labeled ractopamine to the immobilized β2-AR proteins by β-agonists. The IC50 and limit of detection values for... More
