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Acetylcholine protects mesenteric arteries against hypoxia/reoxygenation injury via inhibiting calcium-sensing receptor.

J Pharmacol Sci. 2015; 
Zhao M, He X, Yang YH, Yu XJ, Bi XY, Yang Y, Xu M, Lu XZ, Sun Q, Zang WJ.
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Biochemicals … 3′; CaSR reverse, 5′-TGAAGGTGCAGAGGAAAACC-3′; glyceraldehyde-3-phosphate dehydrogenase (GAPDH) forward, 5′-GGCACAGTCAAGGCTGAGAATG-3′; and GAPDH reverse, 5′-ATGGTGGTGAAGACGCCAGTA-3′ (GenScript, Nanjing, China) … Get A Quote

摘要

The Ca(2+)-sensing receptor (CaSR) plays an important role in regulating vascular tone. In the present study, we investigated the positive effects of the vagal neurotransmitter acetylcholine by suppressing CaSR activation in mesenteric arteries exposed to hypoxia/reoxygenation (H/R). The artery rings were exposed to a modified 'ischemia mimetic' solution and an anaerobic environment to simulate an H/R model. Our results showed that acetylcholine (10(-6) mol/L) significantly reduced the contractions induced by KCl and phenylephrine and enhanced the endothelium-dependent relaxation induced by acetylcholine. Additionally, acetylcholine reduced CaSR mRNA expression and activity when the rings were subjected to 4 ... More

关键词

Acetylcholine; Calcium-sensing receptor; Hypoxia/reoxygenation; Mesenteric artery; Vascular function