| Products/Services Used | Details | Operation |
|---|---|---|
| DNA Sequencing | … chain reaction under the conditions of 4 min at 94 °C, 35 cycles of 60 s at 94 °C, 60 s at 53 °C, 100 s at 72 °C, and a terminal extension for 10 min at 72 °C (Li et al, 2017), and sequenced by Sanger sequencing at Nanjing GenScript Biotechnology Company (Nanjing, China) … | Get A Quote |
This study investigated the biodegradation and metabolic mechanisms of 17β-estradiol (E2) by Novosphingobium sp. ES2-1 isolated from the activated sludge in a domestic sewage treatment plant (STP). It could degrade 97.1% E2 (73.5 μmol/L) in 7 d with a biodegradation half-life of 1.29 d. E2 was initially converted to estrone (E1), then to 4-hydroxyestrone (4-OH-E1), before subsequent monooxygenation reactions cleaved 4-OH-E1 into a metabolite with long-chain ketones structure (metabolite P8). However, when 4-OH-E1 was cleaved through the 4,5-seco pathway, the resulting phenol ring cleavage product could randomly condense with NH3 to yield a pyridine derivative, accompanied by the uncertain loss of a carboxy gr... More
