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Biochemicals> | … PAmCherry and EGFP expression backbones were obtained from Clontech TCRζ-PAmCherry was made as described in [10] CD44-GFP was custom-cloned by Genscript Biotech (Piscataway Township, NJ, USA) 22 Cell Culture and Sample Preparation … | Get A Quote |
: T cell activation is immediately followed by internalization of the T cell receptor (TCR). TCR endocytosis is required for T cell activation, but the mechanisms supporting removal of TCR from the cell surface remain incompletely understood. Here we report that TCR endocytosis is linked to the clathrin-independent carrier (CLIC) and GPI-enriched endocytic compartments (GEEC) endocytic pathway. We show that unlike the canonical clathrin cargo transferrin or the adaptor protein Lat, internalized TCR accumulates in tubules shaped by the small GTPase Cdc42 and the Bin/amphiphysin/Rvs (BAR) domain containing protein GRAF1 in T cells. Preventing GRAF1-positive tubules to mature into endocytic vesicles by expressing ... More