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AEBP1 down regulation induced cell death pathway depends on PTEN status of glioma cells

Sci Rep. 2019; 
Sinha S, Renganathan A, Nagendra PB, Bhat V, Mathew BS, Rao MRS
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ORF cDNA Clones/MolecularCloud PIK3CB ORF clone from GenScript (OHu21708D) was procured to over express PIK3C beta in U138MG cells. Briefly, U138MG cells were transfected with pcDNA-PIK3CB or pcDNA-PTENWT construct or pcDNA3.1+ (negative control) by using Lipofectamine 2000 (Life technologies). Get A Quote

摘要

Glioblastoma (GBM) is the most common aggressive form of brain cancer with overall dismal prognosis (10-12 months) despite all current multimodal treatments. Previously we identified adipocyte enhancer binding protein 1 (AEBP1) as a differentially regulated gene in GBM. On probing the role of AEBP1 over expression in glioblastoma, we found that both cellular proliferation and survival were affected upon AEBP1 silencing in glioma cells, resulting in cell death. In the present study we report that the classical caspase pathway components are not activated in cell death induced by AEBP1 down regulation in PTEN-deficient (U87MG and U138MG) cells. PARP-1 was not cleaved but over-activated under AEBP1 down regulation... More

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