Products/Services Used | Details | Operation |
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DNA Sequencing> | The PCR products were then sequenced using primer-walking strategy from both strands at Genscript Biotech Company at Nanjing, China. | Get A Quote |
We sequenced the complete mitogenome of Atractomorpha sagittaris (Orthoptera: Pyrgomorphidae) and compared with other Acrididea species. The circular genome was 15,763 bp in size and contained the entire set of 37 genes usually present in orthopteran mitogenomes. The AT-skew of this mitogenome was slightly positive (0.17) and the nucleotide composition biased toward A + T nucleotides (73.38%). All of the 13 PCGs initiated with canonical start codon (ATN), except for COI initiating with ACT. In addition, analyses of pairwise genetic distances between individual PCGs in Acrididea species showed that ND1 was the least conserved gene, while COI the most conserved. Furthermore, we reconstructed phylogenetic trees am... More