Products/Services Used | Details | Operation |
---|---|---|
DNA Sequencing> | we usedGenCRISPRTMgRNA/Cas9 lentiCRISPR v2 vector (GenScript, Piscat-away, NJ) targeting the BRCA2 sequence CTGTCTACCTGACCAATCGA. SKOV-3 cells were infected with this vector or an emptyvector. | Get A Quote |
Many chaperones favour binding to hydrophobic sequences that are flanked by basic residues while disfavouring acidic residues. However, the origin of this bias in protein quality control remains poorly understood. Here, we show that while acidic residues are the most efficient aggregation inhibitors, they are also less compatible with globular protein structure than basic amino acids. As a result, while acidic residues allow for chaperone-independent control of aggregation, their use is structurally limited. Conversely, we find that, while being more compatible with globular structure, basic residues are not sufficient to autonomously suppress protein aggregation. Using Hsp70, we show that chaperones with a bia... More