至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Autonomous aggregation suppression by acidic residues explains why chaperones favour basic residues

EMBO J. 2020-03; 
Bert Houben, Emiel Michiels, Meine Ramakers, Katerina Konstantoulea, Nikolaos Louros, Joffré Verniers, Rob van der Kant, Matthias De Vleeschouwer, Nuno Chicória, Thomas Vanpoucke, Rodrigo Gallardo , Joost Schymkowitz , Frederic Rousseau
Products/Services Used Details Operation
DNA Sequencing we usedGenCRISPRTMgRNA/Cas9 lentiCRISPR v2 vector (GenScript, Piscat-away, NJ) targeting the BRCA2 sequence CTGTCTACCTGACCAATCGA. SKOV-3 cells were infected with this vector or an emptyvector. Get A Quote

摘要

Many chaperones favour binding to hydrophobic sequences that are flanked by basic residues while disfavouring acidic residues. However, the origin of this bias in protein quality control remains poorly understood. Here, we show that while acidic residues are the most efficient aggregation inhibitors, they are also less compatible with globular protein structure than basic amino acids. As a result, while acidic residues allow for chaperone-independent control of aggregation, their use is structurally limited. Conversely, we find that, while being more compatible with globular structure, basic residues are not sufficient to autonomously suppress protein aggregation. Using Hsp70, we show that chaperones with a bia... More

关键词

 Hsp70; aggregation; gatekeepers; protein folding.