至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Icariin protects neurons from endoplasmic reticulum stress-induced apoptosis after OGD/R injury via suppressing IRE1α-XBP1 signaling pathway

Life Sci. 2020-05; 
Zhen-Tao Mo, Yu-Ling Liao, Jie Zheng, Wen-Na Li
Products/Services Used Details Operation
ORF cDNA Clones/MolecularCloud … COVID-19 campus closures: see options for getting … Lentiviral expressing plasmid Phs-AVC-IRE1 (GenScript Biotech, China) and the lentiviral packaging plasmids pLP1, pLP2, pLP/VSVG (Invitrogen, USA) were co-transfected and propagated in 293T cells for virus production … Get A Quote

摘要

Icariin (ICA), a flavonol glycoside isolated from Epimedium, has been considered as a potential alternative therapy for ischemic stroke. However, the protective mechanisms of ICA on cerebral ischemia-reperfusion (I/R) are not fully illuminated yet. The effects of ICA on ER stress and inflammatory response which were involved in the pathological process of cerebral I/R were investigated in vitro. Microglia and neurons were subjected to OGD/R. ICA was administrated to microglia 1 h before OGD and maintained 2 h throughout OGD. At 24 h after reoxygenation, the protein expression of IL-1 β, IL-6, TNF-α in the supernatant of microglia was measured using ELISA assay; neuronal apoptosis was assessed by TUNEL stai... More

关键词

Apoptosis, IRE1, Icariin, Neuron, Oxygen-glucose deprivation