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Identification and functional characterization of a novel BEL1-LIKE homeobox transcription factor GmBLH4 in soybean

Plant Cell, Tissue and Organ Culture (PCTOC). 2018-03; 
Yuan Tao, Ming Chen, Yingjie Shu, Yajing Zhu, Shuang Wang, Liyan Huang, Xingwang Yu, Zhankui Wang, Peipei Qian, Weihong Gu & Hao Ma
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Biochemicals … His fusion protein was expressed in Arctic Express strain and induced with 05 mM IPTG for 8 h at 15 °C To investigate the interaction between GmSBH1 and GmBLH4, the purified GST-GmSBH1 protein was bound to Glutathione Sepharose 4B (GenScript, China) for 1 h at … Get A Quote

摘要

The interaction between BEL1- and KNOTTED1-type homeobox transcription factors from TALE (Three-Amino-acid-Loop Extension) class plays key roles in plant growth and development. In our previous study, a soybean KNOTTED1-like gene GmSBH1, which played important roles in soybean growth and development as well as in response to high temperature and humidity (HTH) stress was identified. In the present study, 15 GmSBH1-interacting candidate proteins were identified by yeast two-hybrid system. From the 15 candidate proteins, a novel BEL1-LIKE homeodomain 4 protein, GmBLH4, was selected to isolate and characterize through sequence alignment, phylogenetic analysis and subcellular localization. GmBLH4 showed tissues spe... More

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