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Molecular Biology Tools> | The amino acid sequence, protein molecular weight, isoelectric point, stability index, and hydrophobicity of the BocZ-ISO gene were analyzed and predicted by ExPASy (http://web.expasy.org) and NCBI (https://www.ncbi.nlm.nih.gov/). Subcellular localization was predicted by WoLF PSORT (http://www.genscript.com/wolf-psort.html). The conserved domain were predicted by NCBI (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi). Phylogenetic tree analysis of the Z-ISO proteins was executed in MEGA 6.0 using the neighbor-joining (NJ) method. | Get A Quote |
Ζ-carotene isomerase (Z-ISO) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. capitata Z-ISO (BocZ-ISO) gene sequence was obtained from Brassica database (BRAD), and preformed for bioinformatics analysis. The BocZ-ISO gene mapped to chromosomes 5, and contains an open reading frame of 1,092 bp that encodes a 363-amino acid protein with a calculated molecular mass of 40.24 kD and an isoelectric point (pI) of 9.56. Subcellular localization predicted the BocZ-ISO gene was in the chloroplast. The conserved domain of the BocZISO protein is COG4094. The Z-ISO protein is most closely related to B. napus and B. rapa. The findings of the present study provide a molecular basis for the... More