Products/Services Used | Details | Operation |
---|---|---|
Molecular Biology Tools> | ...The results were normalized with the housekeeping gene mouse-Actin. Primer sequences were designed using online tools from GenScript Corporation. For human IL-6 they were: forward 5'-AATGAGGAGACTTGCCTGGT-3', reverse 5'-GCAGGAACTGGATCAGGACT-3'. Human IL-8... | Get A Quote |
Two parallel interleukin-1 (IL-1)-mediated signaling pathways have been uncovered for IL-1R-TLR-mediated NFkappaB activation: TAK1-dependent and MEKK3-dependent pathways, respectively. The TAK1-dependent pathway leads to IKKalpha/beta phosphorylation and IKKbeta activation, resulting in classic NFkappaB activation through IkappaBalpha phosphorylation and degradation. The TAK1-independent MEKK3-dependent pathway involves IKKgamma phosphorylation and IKKalpha activation, resulting in NFkappaB activation through dissociation of phosphorylated IkappaBalpha from NFkappaB without IkappaBalpha degradation. IL-1 receptor-associated kinase 4 (IRAK4) belongs to the IRAK family of proteins and plays a critical role in IL-... More