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Involvement of miR-27a-3p in diabetic nephropathy via affecting renal fibrosis, mitochondrial dysfunction, and endoplasmic reticulum stress

J Cell Physiol. 2020-07-01; 
Lina Wu, Qingzhu Wang, Feng Guo, Xiaojun Ma, Jiao Wang, Yanyan Zhao, Yushan Yan, Guijun Qin
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Mutant Libraries … mice (35–45 g) and nondiabetic db/m mice (C57BLKS/J background, 17–23 g) were purchased from Chang Sheng biotechnology Co., Ltd … Both wild type (WT) and mutant type (MUT) 3′‐UTR of prohibitin or TMBIM6 were synthesized in Genscript Biotech (Nanjing, China) and … Get A Quote

摘要

Diabetic nephropathy (DN) is acknowledged as a serious chronic complication of diabetes mellitus. Nevertheless, its pathogenesis is complicated and unclear. Thus, in this study, the role of miR-27a-3p-prohibitin/TMBIM6 signaling axis in the progression of DN was elucidated. Type 2 diabetic db/db mice and high glucose (HG)-challenged HK-2 cells were used as in vivo and in vitro models. Our results showed that miR-27a-3p was upregulated and prohibitin or transmembrane BAX inhibitor motif containing 6 (TMBIM6) was downregulated in the kidney tissues of db/db mice and HG-treated HK-2 cells. Silencing miR-27a-3p enhanced the expression of prohibitin and TMBIM6 in the kidney tissues and HK-2 cells. Inhibition of miR-... More

关键词

Diabetic nephropathy, TMBIM6, endoplasmic reticulum stress, miR-27a-3p, mitochondrial dysfunction, prohibitin