至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Identification of a novel methyltransferase-type 12 protein from Haemonchus contortus and its effects on functions of goat PBMCs

Parasit Vectors. 2020-03-01; 
Muhammad Ehsan, Javaid A Gadahi, Tingqi Liu, Mingmin Lu, Yujian Wang, Muhammad W Hasan, Muhammad Haseeb, Ruofeng Yan, Lixin Xu, Xiaokai Song, Xing-Quan Zhu, Xiangrui Li
Products/Services Used Details Operation
DNA Sequencing … TransGen Biotech, Beijing, China). The amplified MTF-12 gene was subjected to restriction endonuclease digestion with EcoRI and SacI, and sequenced by GenScript (Nanjing, Jiangsu, China). Subsequently, the identified … Get A Quote

摘要

background: Methyltransferases (MTFs) are broad range of enzymes, which are ubiquitously expressed in diverse organisms ranging from bacteria to animals. MTFs proteins have been associated with various biological/cellular processes including transcriptional regulation, subcellular protein and RNA localization, signal transduction and DNA-damage repair. However, the role of MTFs in immune mechanism during host-parasite interaction has not been addressed yet. results: An open reading frame (764 bp) of methyltransferase-type 12 gene of H. contortus denoted as HcMTF-12, was successfully cloned using reverse transcriptase-polymerase chain reaction (RT-PCR) followed by prokaryotic expression in Escherichia coli BL21 ... More

关键词

Goat, Haemonchus contortus, Host–parasite interactions, Methyltransferase-type 12, PBMC