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Cryo-EM reconstruction of a VPS13 fragment reveals a long groove to channel lipids between membranes

J Cell Biol. 2020-05-01; 
PeiQi Li, Joshua Aaron Lees, C Patrick Lusk, Karin M Reinisch
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GenParts™ DNA Fragments … L827E). Coding sequences for fragments of the genome incorporating the mutations were purchased from Genscript and replaced the VPS13 WT sequence in the mutant constructs. Fluorescence microscopy. Yeast expressing … Get A Quote

摘要

A single particle cryo-EM reconstruction of an ∼160-kD N-terminal fragment of the lipid transport protein VPS13 reveals an ∼160-Å long channel lined with hydrophobic residues suitable for solubilizing multiple lipid fatty acid moieties. The structure suggests that VPS13 and related proteins, like the autophagy protein ATG2, can act as bridges between organelle membranes to allow bulk lipid flow between organelles.

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