| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | … The CloneEZ ® PCR Cloning Kit (GenScript, Piscataway, NJ, USA) was used to assemble the gh1-1 and pRS424 PCR products. All vectors were introduced into E. coli Top10 and the transformants were selected on Luria–Bertani agar medium containing 100 µg/mL ampicillin … | Get A Quote |
In a previously engineered recombinant, the cellobiose fermentation rate was significantly lower than the glucose fermentation rate. Thus, we implemented a genome-wide perturbation library to find gene targets for improving the cellobiose fermentation capability of the yeast strain. Unexpectedly, we discovered a transformant that contained an additional β-glucosidase gene (-), possibly through homologous recombination between the plasmids. The additional β-glucosidase led to the fastest cellobiose fermentation activity among all the transformants evaluated, and the strain demonstrated significantly higher β-glucosidase activity than the control strain, especially during the initial exponential growth phase.... More
