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Copper-Induced Inactivation of Camel Liver Glutathione S-Transferase

Biol Trace Elem Res. 2015-06-01; 
Anwar Ahmed, Ajamaluddin Malik, Haseeb Jagirdar, Nayyar Rabbani, Mohd Shahnawaz Khan, Abdulrahman M Al-Senaidy, Mohamed A Ismael
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ORF cDNA Clones/MolecularCloud … Ltd), DTNB (Sigma), Chirascan-plus CD Spectrometer (Applied Photophysics), Fluorescence Spectrometer F-750 (Jasco, Inc.), Ultrospec 2100 pro Spectrophotometer (Amersham Biosciences), pH meter (Sentron), Thermomix (Eppendorf), and SDS-PAGE (GenScript) … Get A Quote

摘要

Glutathione S-transferases (GSTs) are multifunctional enzymes and play an important role in detoxification of xenobiotics and protection against oxidative stress. Camel liver glutathione transferase (cGST) was recently isolated and characterized in our lab. In this study, we have evaluated the effect of monovalent, divalent, and trivalent cations on its activity and stability. Cu(++) was found to be the potent inhibitor of GST activity which loses complete activity at 0.5-mM concentration. Other metal ions did not inhibit GST even at higher concentration of 2 mM. GST incubated with Cu(++) (0.1 mM) resulted decrease in free sulfhydryl groups by 55%, whereas other metal ions did not show any effect on free thiol ... More

关键词

Aggregation, Copper, Glutathione S-transferases, Metal ions, Thiol groups