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GenParts™ DNA Fragments> | … To recover ZIKV variants we used infectious subgenomic amplicons (ISA) [29,33,34] as previously described [7]. Recoded fragments were de novo synthesized (GenScript, Piscataway, NJ, USA), amplified with high fidelity PCR (Invitrogen Platinum PCR SuperMix, High Fidelity … | Get A Quote |
We studied whether cytosine phosphate-guanine (CpG) recoding in a viral genome may provide oncolytic candidates with reduced infection kinetics in nonmalignant brain cells, but with high virulence in glioblastoma stem cells (GSCs). As a model, we used well-characterized CpG-recoded Zika virus vaccine candidates that previously showed genetic stability and safety in animal models. In vitro, one of the CpG-recoded Zika virus variants had reduced infection kinetics in nonmalignant brain cells but high infectivity and oncolytic activity in GSCs as represented by reduced cell proliferation. The recoded virus also efficiently replicated in GSC-derived tumors in ovo with a significant reduction of tumor growth. We als... More