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A SARS-CoV-2 surrogate virus neutralization test based on antibody-mediated blockage of ACE2–spike protein–protein interaction

Nature Biotechnology. 2020-07; 
Chee Wah Tan, Wan Ni Chia, Xijian Qin, Pei Liu, Mark I.-C. Chen, Charles Tiu, Zhiliang Hu, Vivian Chih-Wei Chen, Barnaby E. Young, Wan Rong Sia, Yee-Joo Tan, Randy Foo, Yongxiang Yi, David C. Lye, Danielle E. Anderson & Lin-Fa Wang
Products/Services Used Details Operation
Serology SARS-Cov-2 detection kits The SARS-CoV-2 N and S and SARS-CoV N genes were synthesized by GenScript and BioBasic. The SARS-CoV-2 N and SARS-CoV N genes were cloned into the pcDNA3...The recombinant SARS-CoV-2 RBD and S1 and SARS-CoV RBD proteins were produced by GenScript using the baculovirus-insect cell expression system...For performance comparison, recombinant SARS-CoV-2 RBD was also produced by GenScript in the mammalian expression system. Get A Quote

摘要

A robust serological test to detect neutralizing antibodies to SARS-CoV-2 is urgently needed to determine not only the infection rate, herd immunity and predicted humoral protection, but also vaccine efficacy during clinical trials and after large-scale vaccination. The current gold standard is the conventional virus neutralization test requiring live pathogen and a biosafety level 3 laboratory. Here, we report a SARS-CoV-2 surrogate virus neutralization test that detects total immunodominant neutralizing antibodies targeting the viral spike (S) protein receptor-binding domain in an isotype- and species-independent manner. Our simple and rapid test is based on antibody-mediated blockage of the interaction betwe... More

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