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Potassium content diminishes in infected cells of Medicago truncatula nodules due to the mislocation of channels MtAKT1 and MtSKOR/GORK

J Exp Bot. 2020-11; 
Elena E Fedorova, Teodoro Coba de la Peña, Victoria Lara-Dampier, Natalia A Trifonova, Olga Kulikova, José J Pueyo, M Mercedes Lucas
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Monoclonal Antibody Services … used. The immunolocalization was carried out with the custom made Ab developed in rabbit (GenScript) at dilution of 1:50, followed by Colloidal 10 nm gold conjugated Goat anti-Rabbit Ab (BioCell). Samples … Microscopy Service in ICA … Get A Quote

摘要

Rhizobia establish a symbiotic relationship with legumes that results in the formation of root nodules, where bacteria encapsulated by a membrane of plant origin (symbiosomes), convert atmospheric nitrogen into ammonia. Nodules are more sensitive to ionic stresses than the host plant itself. We hypothesize that such a high vulnerability might be due to defects in ion balance in the infected tissue. Low temperature-scanning electron microscopy (LTSEM) and X-ray microanalysis of Medicago truncatula nodules revealed a potassium (K+) decrease in symbiosomes and vacuoles during the lifespan of infected cells. To clarify K+ homeostasis in the nodule, we performed phylogenetic and gene expression analyses, confocal an... More

关键词

Medicago truncatula, AKT1, GORK, SKOR, X-ray microanalysis, potassium channels, symbiosis, symbiosome