| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | … 2 Materials. 2.1 ChR Plasmid DNA (pDNA). cDNAs of … examples. With the sequence information, the ChR gene can also be synthesized with codon optimization for improving expression from companies, such as GenScript. Many … | Get A Quote |
Channelrhodopsin (ChR)-based optogenetics is one promising approach to restore vision in photoreceptor degenerative diseases such as retinitis pigmentosa (RP) and age-related macular degeneration (AMD). Currently, a large number of ChRs from different alga species as well as engineered variants are available. They vary with their light response properties like peak sensitive wavelength (λ), current amplitude, and kinetics. Therefore, it is important to choose an appropriate ChR for practical applications, such as vision restoration. Here we describe a standard laboratory protocol for characterizing properties of ChRs in in vitro in human embryonic kidney (HEK) cells. Based on such characterization, we also dis... More
