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Genome-wide analyses of direct target genes of an ERF11 transcription factor involved in plant defense against bacterial pathogens

Biochem Biophys Res Commun. 2020; 
Juan Wu, Yong Deng, Junhe Hu, Chenzhong Jin, Xiwu Zhu, Defang Li
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DNA Sequencing … was performed overnight with Protein A Dynabeads (novex) which had been previously bound to monoclonal anti-c-Myc (GenScript) … ChIP-Seq libraries were prepared and sequenced on a Hiseq- PE150 sequencing system (Illumina) by Beijing Novogene Company. 2.3 … Get A Quote

摘要

Ethylene responsive factor ERF11 containing the ERF-associated amphiphilic repression (EAR) motif enhances plant resistance to bacterial pathogens. However, the underlying molecular mechanisms regulated by transcription factor ERF11 are poorly understood, in tobacco or other model plants. Here, we revealed the genome-wide binding landscape of BrERF11b in Nicotiana benthamian by conducting chromatin immunoprecipitation experiments followed by high-throughput sequencing (ChIP-seq) and bioinformatic analyses. Our results also revealed a GCCbox-like consensus BrERF11b-binding DNA motif: VCGCCGCC. By further integrative analysis of ChIP-seq and RNA-seq data, and the confirmation of electrophoretic mobility shift ass... More

关键词

Bacterial pathogens, ChIP-seq, ERF11, ERF4, NIMIN2, TAF15b