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Long Noncoding RNA Expression Profiling Reveals Upregulation of Uroplakin 1A and Uroplakin 1A Antisense RNA 1 under Hypoxic Conditions in Lung Cancer Cells

Mol Cells. 2020-12; 
Yuree Byun, Young-Chul Choi, Yongsu Jeong, Jaeseung Yoon, Kwanghee Baek
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Mutagenesis Services … μg) were separated on 4% to 12% precast protein gels (Koma Biotech, Korea) and … C-4) were purchased from BD Biosciences (USA) and Santa Cruz Biotechnology (USA), respectively … pcDNA3.1-UPK1A-AS1-Wild and pcDNA-UPK1A-AS1-Mutant, respectively (GenScript, USA … Get A Quote

摘要

Hypoxia plays important roles in cancer progression by inducing angiogenesis, metastasis, and drug resistance. However, the effects of hypoxia on long noncoding RNA (lncRNA) expression have not been clarified. Herein, we evaluated alterations in lncRNA expression in lung cancer cells under hypoxic conditions using lncRNA microarray analyses. Among 40,173 lncRNAs, 211 and 113 lncRNAs were up- and downregulated, respectively, in both A549 and NCI-H460 cells. Uroplakin 1A () and -antisense RNA 1 (), which showed the highest upregulation under hypoxic conditions, were selected to investigate the effects of on the expression of and the mechanisms of hypoxia-inducible expression. Following transfection of cells wit... More

关键词

hypoxia, hypoxia-inducible factor 1α, microarray, uroplakin 1A, uroplakin 1A antisense RNA 1