| Products/Services Used | Details | Operation |
|---|---|---|
| Plasmid DNA Preparation | … No.: HAF018). GenScript (Piscataway, NJ, USA). pcDNA3.1+ C-eGFP-TP plasmid (custom-made). QIAGEN (Germantown, MD, USA). Plasmid Miniprep kit (Cat No.: 10043). Research Products International Corp (Mount Prospect … | Get A Quote |
The limited amount of fusion protein transported into cytosol milieu has made it challenging to obtain a sufficient amount for further applications. To avoid the laborious and expensive task, T7 promoter-driving pET-30a(+) coding for chimeric gene of thymidine phosphorylase and core streptavidin as a model system was constructed and transformed into a variety of strains with T7 expression system. Our results demonstrated that the pET-30a(+)-TP-coreSA/Lemo21(DE3) system is able to provide efficient expression of soluble TP-coreSA fusion protein for purification. Moreover, the eluted TP-coreSA fusion protein tethered on biotinylated A549 carcinoma cells could effectively eliminate these malignant cells after adm... More
