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Generating broad-spectrum tolerance to ALS-inhibiting herbicides in rice by base editing

Sci China Life Sci. 2020-11; 
Rui Zhang, Sha Chen, Xiangbing Meng, Zhuangzhuang Chai, Delin Wang, Yuge Yuan, Kunling Chen, Linjian Jiang, Jiayang Li, Caixia Gao
Products/Services Used Details Operation
Synthetic sgRNA and crRNA Service … pH-BE3, was made as follows. APOBEC partial nCas9 and UGI sequences without Bsa I were synthesized commercially (GenScript, Nanjing, China) and cloned into pUC57 as an intermediate vector. The other part of nCas9 … Get A Quote

摘要

Herbicide-tolerant rice varieties generated by genome editing are highly desirable for weed control. We have used a cytosine base editor to create a series of missense mutations in the P171 and/or G628 codons of the acetolactate synthase (ALS) gene to confer herbicide tolerance in rice. The four different missense mutations in the P171 codon, P171S, P171A, P171Y and P171F, exhibited different patterns of tolerance towards five representative herbicides from five chemical families of ALS inhibitors. For example, P171S and P171A had lower levels of tolerance than P171Y and P171F to bispyribac but not to the other herbicides. Interestingly, a novel triple mutant (P171F/G628E/G629S) had the highest tolerance to all... More

关键词

acetolactate synthase (ALS), base editing, herbicide tolerance, rice