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Crystal structures of ORFV125 provide insight into orf virus-mediated inhibition of apoptosis

Biochem J. 2020; 
Chathura D Suraweera, Mark G Hinds, Marc Kvansakul
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ORF cDNA Clones/MolecularCloud … Synthetic cDNA encoding for codon-optimized ORFV125 (Uniprot Accession number W6EVU4) lacking the 30 C-terminal residues was cloned into the bacterial expression vector pGex-6p-1 (GenScript) and is subsequently referred to as ORFV125 … Get A Quote

摘要

Premature apoptosis of cells is a strategy utilized by multicellular organisms to counter microbial threats. Orf virus (ORFV) is a large double-stranded DNA virus belonging to the poxviridae. ORFV encodes for an apoptosis inhibitory protein ORFV125 homologous to B-cell lymphoma 2 or Bcl-2 family proteins, which has been shown to inhibit host cell encoded pro-apoptotic Bcl-2 proteins. However, the structural basis of apoptosis inhibition by ORFV125 remains to be clarified. We show that ORFV125 is able to bind to a range of peptides spanning the BH3 motif of human pro-apoptotic Bcl-2 proteins including Bax, Bak, Puma and Hrk with modest to weak affinity. We then determined the crystal structures of ORFV125 alone ... More

关键词

Bcl-2, apoptosis, crystallography, orf virus, poxvirus, virology