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Flexible loops of New Delhi metallo-β-lactamase modulate its activity towards different substrates

Int J Biol Macromol. 2020; 
Joanna E Raczynska, Barbara Imiolczyk, Marlena Komorowska, Joanna Sliwiak, Justyna Czyrko-Horczak, Krzysztof Brzezinski, Mariusz Jaskolski
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Mutagenesis Services … NDM-1. 2. Materials and methods. 2.1. Protein expression and purification. Gene sequences, cloned into pET-15b expression vector, were obtained from GenScript, initially by chemical synthesis and then mutagenesis. All sequences … Get A Quote

摘要

Two accessory loop regions that are present in numerous variants of New Delhi metallo-β-lactamases (NDM) are important for the enzymatic activity. The first one is a flexible loop L3 that is located near the active site and is thought to play an important role in the catalytic process. The second region, Ω loop is located close to a structural element that coordinates two essential zinc ions. Both loops are not involved in any specific interactions with a substrate. Herein, we investigated how the length and hydrophobicity of loop L3 influence the enzymatic activity of NDMs, by analyzing mutants of NDM-1 with various deletions/point mutations within the L3 loop. We also investigated NDM variants with sequence... More

关键词

Antibiotic resistance, Crystal structure, NDM metallo-β-lactamase (MBL)