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Rational design of in situ localization solid-state fluorescence probe for bio-imaging of intracellular endogenous cysteine

Talanta. 2020; 
Ying Liu, Yong-Xiang Wu, Dailiang Zhang, Hongmei Zhong, Dian Li, Kangdi He, Wen-Ting Wei, Shengrong Yu
Products/Services Used Details Operation
PCR Cloning and Subcloning … FU-HaloTag-Turq2-Wm. Cloning and custom gene synthe- sis was done by Genscript (Piscataway NJ, US). Positioning the HaloTag at the N-terminus was based on the literature (eg 42) and on the structure of the protein of interest … Get A Quote

摘要

Fluorescence detection technology has been widely concerned for its advantages of low cost, simple operation, good sensitivity, real-time and non-destructive biological imaging. However, most fluorophores emit bright fluorescence in solution, and the fluorescence decreases significantly in the high concentration or solid/aggregated state, which is called aggregation-caused quenching (ACQ). Cysteine (Cys) is an important kind of amino-acid in the field of bio-medicine, whose main function is to participate in metabolism and protein synthesis, detoxification, but intracellular cysteine concentrations (30-200 μM) are much low, and direct detection of endogenous cysteine is hampered by interference with other thi... More

关键词

Bio-imaging, Excited-state intramolecular proton transfer, In situ localization, Intracellular endogenous cysteine, Solid-state fluorescence probe