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Enolase-specific cross antibodies induce neutrophilic inflammation in the intestine

J Leukoc Biol. 2020; 
Jianli Lin, Bai-Sui Feng, Nana Huang, Na Ma, Zhi-Qiang Liu, Qing Shu, Nanshan Zhong, Zhi-Gang Liu, Ping-Chang Yang
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Custom RNAi Services … St. Louis, MO). RNAi kits of enolase and csp9 were provided by GenScript (Nanjing, China). Abs of CD11b (BUV563) and Ly‐6G (APC‐cy7) for flow cytometry were purchased from BD Biosciences (Franklin Lakes, NJ). Reagents … Get A Quote

摘要

The pathogenesis of ulcerative colitis (UC) is to be further investigated. House dust mites (HDM) are highly associated with the pathogenesis of immune inflammation in the body. This study aims to investigate the role of enolase (one of the HDM-derived proteins)-specific cross Abs in the induction of UC-like inflammation. The enolase specific IgG (EsIgG) was identified in UC patients by mass spectrometry. Mice were treated with EsIgG to induce inflammation in the colon mucosa. EsIgG was detected in the serum and the colon tissues of UC patients, which was positively correlated with the polymorphonuclear neutrophil (PMN) counts in the blood and colon tissues of UC patients. EsIgG formed immune complexes with the... More

关键词

house dust mites, inflammation, intestine, neutrophil, ulcerative colitis