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The Cytokine IL-1β and Piperine Complex Surveyed by Experimental and Computational Molecular Biophysics

Biomolecules. 2020; 
Gabriel Zazeri, Ana Paula Ribeiro Povinelli, Marcelo de Freitas Lima, Marinônio Lopes Cornélio
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ORF cDNA Clones/MolecularCloud … (Schnelldorf, Bavaria, Germany), as dibasic sodium phosphate (>99%) reagents, anhydrous citric acid (>99%), and sodium chloride (>99%). Lyophilised IL-1β (>97%) was purchased from GenScript Biotech (Piscataway, Nova Jersey, EUA) … Get A Quote

摘要

The bioactive piperine, a compound found in some pepper species, has been widely studied because of its therapeutic properties that include the inhibition of an important inflammation pathway triggered by interleukin-1 beta (IL-1β). However, investigation into the molecular interactions between IL-1β and piperine is not reported in the literature. Here, we present for the first time the characterisation of the complex formed by IL-1β and piperine through experimental and computational molecular biophysical analyses. Fluorescence spectroscopy unveiled the presence of one binding site for piperine with an affinity constant of 14.3 × 10 M at 298 K. The thermodynamic analysis indicated that the interaction with... More

关键词

IL-1β, cytokine, fluorescence spectroscopy, molecular docking, piperine, umbrella sampling