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Developing an endogenous quorum-sensing based CRISPRi circuit for autonomous and tunable dynamic regulation of multiple targets in Streptomyces

Nucleic Acids Research. 2020-07;

Tian J, Yang G, Gu Y, Sun X, Lu Y, Jiang W

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Endotoxin Detection & Removal System	of the GBL-responsive promoter srbAp or the strong constitutive promoter ermEp* (GenScript, Nanjing sgRNAs (targeting fabH3, gltA2 and cm2) for multiplex gene repression were constructed by GenScript	Get A Quote