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Custom DNA/RNA Oligos> | … Briefly, HeyA8 and Kuramochi cells were transiently transfected with 5′FAM-labeled oligonucleotides (n = 6, Table S7) synthesized by GenScript (Piscataway, NJ, USA) and after 24h of transfection, reseeded in eight-well chamber slides (ibidi USA, Madison, WI, USA) and … | Get A Quote |
Fragile X-related protein-1 (FXR1) gene is highly amplified in patients with ovarian cancer, and this amplification is associated with increased expression of both FXR1 mRNA and protein. FXR1 expression directly associates with the survival and proliferation of cancer cells. Surface sensing of translation (SUnSET) assay demonstrates that FXR1 enhances the overall translation in cancer cells. Reverse-phase protein array (RPPA) reveals that cMYC is the key target of FXR1. Mechanistically, FXR1 binds to the AU-rich elements (ARE) present within the 3' untranslated region (3'UTR) of cMYC and stabilizes its expression. In addition, the RGG domain in FXR1 interacts with eIF4A1 and eIF4E proteins. These two interactio... More