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Plasmid DNA Preparation> | … CTNNBIP1 overexpression plasmid was constructed by GenScript Co., Ltd. (Nanjing, China). HAM-3 cells were cultured overnight at 37C in a six-well plate with BML-284 (10 μM, Selleck Chemicals in China, Shanghai, China) and transfected with miR-29a-3p mimics, inhibitors, … | Get A Quote |
background: Ameloblastoma (AB) is a common epithelial odontogenic tumor. The Wnt/β-catenin pathway has been found to be related to AB invasion. methods: The alteration expression of microRNAs (miRNAs) and messenger RNAs (mRNAs) was performed by miRNA and mRNA microarray analysis and validated by quantitative real-time polymerase chain reaction (RT-PCR). The effects of miR-29a-3p on migration and invasion in AB cells were evaluated by a transwell assay. Bioinformatic prediction was conducted using the miRSystem and validated by quantitative RT-PCR, western blot, and a luciferase reporter assay. results: miR-29a-3p was overexpressed in AB tissues, which promoted the migration and invasion of AB cells in vitro. C... More