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DNA Sequencing> | … The primers used in this study are listed in Table S2 and were synthesized by Ykang (Zhejiang, China). The glycosyltransferases module (gtfABC) and glycosyl synthesis module (evaAEBD) were synthesized by Genscript (Nanjing, China), according to the sequence of the … | Get A Quote |
Oritavancin is a new-generation semisynthetic lipoglycopeptide antibiotic used to prevent the spread of vancomycin-resistant Gram-positive bacteria. The glycopeptide A82846B is the direct precursor of oritavancin. Considering the structural similarity between A82846B and vancomycin, the vancomycin producer was used as a chassis for the construction of a strain producing high-quality A82846B. To construct the A82846B synthetic pathway, we established a highly efficient CRISPR-Cas12a system by optimizing the conditions of conjugation and by screening the regulatory elements in the , which is difficult to be genetically manipulated. The efficiency of gene knockout was almost 100%. The glycosyltransferases module ... More