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Custom Vector Construction> | … GenScript Inc(China), designated as OP-LpNES1(Genebank Accession NO: MZ190022). The auxotrophic marker gene LEU2 was amplified from vector pRS605(stored in our lab), upstream and downstream homologous region of the GAL80 gene (indicted as 80up and 80down … | Get A Quote |
Laggera pterodonta, known in China as 'Choulingdan' for its stimulous odor, has long been used as traditional herbal medicine. The essential oil of L. pterodonta, which exhibits various pharmacological activities, is a rich resource of monoterpenes and sesquiterpenes. To date, however, the terpene synthases responsible for their production remain unknown. In present study, a new terpene synthase gene (LpNES1) was identified from L. pterodonta, transcript level of which was significantly upregulated in response to methyl jasmonate treatment. Recombinant LpNES1 could synthesize (E)-nerolidol and minor β-farnesene from farnesyl diphosphate and linalool from geranyl diphosphate in vitro. Whereas, only sesquiterpen... More