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Tuning Gene Expression by Phosphate in the Methanogenic Archaeon

ACS Synth Biol. 2021-10; 
Taiwo S Akinyemi, Nana Shao, Zhe Lyu, Ian J Drake, Yuchen Liu, William B Whitman
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Bacterial Expression … limiting, resulting in the upregulation of genes controlled by Ppst. As expected, when the mCherry gene was under the control of the … , pMEV5mT-P93-BRE1-3, and pMEV5mT-P93-UTR1-5 were all synthesized by GenScript. These plasmids were cloned into E. coli Top10. … Get A Quote

摘要

is a rapidly growing, hydrogenotrophic, and genetically tractable methanogen with unique capabilities to convert formate and CO to CH. The existence of genome-scale metabolic models and an established, robust system for both large-scale and continuous cultivation make it amenable for industrial applications. However, the lack of molecular tools for differential gene expression has hindered its application as a microbial cell factory to produce biocatalysts and biochemicals. In this study, a library of differentially regulated promoters was designed and characterized based on the promoter, which responds to the inorganic phosphate concentration in the growth medium. Gene expression increases by 4- to 6-fold whe... More

关键词

5′ untranslated region, Methanococcus maripaludis, gene regulation, heterologous gene expression, phosphate, promoter