| Products/Services Used | Details | Operation |
|---|---|---|
| PCR and Cloning | … inhibitors and 1.0 µl MMLV reverse transcriptase according to the manufacturer’s instructions (Genscript, Piscataway, NJ, USA). RT-PCR was carried out in a 20 µl reaction using 1 U of Taq DNA polymerase (Genscript, Piscataway NJ, USA), 1× Taq buffer, 0.2 mM dNTPs, 5.0 µM … | Get A Quote |
Next-generation sequencing is a robust approach to sequence plant virus genomes in a very short amount of time compared to traditional sequencing methods. Maize dwarf mosaic virus (MDMV) is one of the most important plant viruses worldwide and a significant threat to maize production. In this study, we sequenced 19 MDMV isolates (10 from Johnsongrass and 9 from maize) collected in Oklahoma and Missouri during 2017-2019 using Illumina sequencing and determined the genetic diversity. Sequence reads were assembled and 19 nearly complete genome sequences of MDMV isolates were obtained. Phylogenetic analysis based on complete genomes nucleotide and amino acid sequences revealed two main clusters and a close evolutio... More
