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Multiple strategies for metabolic engineering of Escherichia coli for efficient production of glycolate

Biotechnol Bioeng. 2021-09; 
Tong Zhu, Die Yao, Di Li, Hongtao Xu, Shiru Jia, Changhao Bi, Jun Cai, Xinna Zhu, Xueli Zhang
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GenParts™ DNA Fragments … Then, the primer pair ycdW-TK-up-T7/ycdW-TK-down-T7 was used to amplify the T7-ycdW DNA fragment for the second recombination step, using the plasmid DNA of pUC57-T7 (synthesized by GenScript, China) as the template. The correct colonies verified by PCR were … Get A Quote

摘要

Glycolate is a bulk chemical with wide applications in the textile, food processing, and pharmaceutical industries. Glycolate can be produced from glucose via the glycolysis and glyoxylate shunt pathways, followed by reduction to glycolate. However, two problems limit the productivity and yield of glycolate when using glucose as the sole carbon source. The first is a cofactor imbalance in the production of glycolate from glucose via the glycolysis pathway, since NADPH is required for glycolate production, while glycolysis generates NADH. To rectify this imbalance, the NADP -dependent glyceraldehyde 3-phosphate dehydrogenase GapC from Clostridium acetobutylicum was introduced to generate NADPH instead of NADH in... More

关键词

Escherichia coli, NADP+-dependent GAPDH, g lycolate, glucose