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Generation of stable cell lines using readthrough expression from lentiviral integration

Biotechnol Lett. 2021-09; 
Anish Jadav, Kevin Truong
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GenParts™ DNA Fragments … plasmids was done by Genscript. RCaMP was synthesized as a fusion with the plasma membrane localization peptide of Lyn kinase ( 1 MGCIKSKGKDSA 12 ). Human VEGFR2 (amino acids 1–1356), Venus and Cerulean were synthesized by Genscript. Synthesize fragments … Get A Quote

摘要

Lentiviral infection is often used to integrate genetic material into cells to stably express transgenes of interest. Depending on the location of integration into the host genome, readthrough expression of the lentiviral cargo can occur via an upstream endogenous promoter, which is typically an unwanted phenomenon because it can result in dysfunctional expression. The purpose of this study was to demonstrate that readthrough expression can be a wanted phenomenon for expressing functional proteins while at the same time reducing the size of the lentiviral transfer plasmid. Readthrough expression was used to generate HEK293 cell lines stably expressing fluorescent reporter proteins, reporter protein-antibiotic r... More

关键词

Cell-based therapy, Gene expression, Lentivirus, Promoter, Readthrough