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Custom Vector Construction> | … ORF clone (Genbank accession number: NM_003563) were purchased from GenScript and were sub-cloned into pcDNA3.1 vector with a … Cullin3 ORF clone (Genbank accession number: NM_003590) was purchased from GenScript and was sub-cloned into pcDNA3.1 vector … | Get A Quote |
Programmed death ligand 1 (PD-L1) is critical for the ability of cancer cells to evade attacks by the host immune system. However, the molecular mechanisms controlling PD-L1 expression have not been fully understood. Here, we demonstrate that sorting nexin 6 (SNX6) is a novel regulator of PD-L1 expression. Knockdown of SNX6 in cancer cells significantly decreases PD-L1 protein levels. In contrast, loss of SNX6 does not reduce PD-L1 mRNA levels. Instead, SNX6 interacts with Cullin3, an E3 ubiquitin ligase responsible for PD-L1 ubiquitination and subsequent degradation. By binding with Cullin3, SNX6 decreases the interaction between the adaptor protein speckle-type POZ protein and Cullin3, which in turn downregul... More