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Context-Specific Function of the Engineered Peptide Domain of PHPB

J Virol. 2021-08; 
R Alexander Martino, Edwin C Fluck, Jacqueline Murphy, Qiang Wang, Henry Hoff, Ruth A Pumroy, Claudia Y Lee, Joshua J Sims, Soumitra Roy, Vera Y Moiseenkova-Bell, James M Wilson
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Recombinant Antibody Expression … Ly6a-hIgG1 recombinant protein was produced on a fee-for-service basis by Genscript Biotech 367 … Genscript synthesized and cloned the DNA sequence for the fusion protein into a 368 … Genscript cultured cells for six days after transfection in a 370 … Get A Quote

摘要

One approach to improve the utility of adeno-associated virus (AAV)-based gene therapy is to engineer the AAV capsid to (i) overcome poor transport through tissue barriers and (ii) redirect the broadly tropic AAV to disease-relevant cell types. Peptide- or protein-domain insertions into AAV surface loops can achieve both engineering goals by introducing a new interaction surface on the AAV capsid. However, we understand little about the impact of insertions on capsid structure and the extent to which engineered inserts depend on a specific capsid context to function. Here, we examine insert-capsid interactions for the engineered variant AAV9-PHP.B. The 7-amino-acid peptide insert in AAV9-PHP.B facilitates trans... More

关键词

AAV, Ly6a, PHP.B, adeno-associated virus, capsid engineering, receptor-mediated transcytosis