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Construction and preservation of a stable and highly expressed recombinant Helicobacter pylori vacuolating cytotoxin A with apoptotic activity

BMC Microbiol. 2021-08; 
Ling-Zhi Yuan, Xiao Shi, Dan Tang, Shao-Peng Zheng, Zhi-Ming Xiao, Fen Wang
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Nucleic Acid Purification & Analysis … The membrane was washed with 1 × PBST (1× phosphate buffered saline (PBS), 0.06% Tween-20, pH 7.4) buffer twice, for 5 min each time, removed and sealed with rapid sealing liquid (Genscript Biotech (Nanjing) Corp Co., Ltd) for 8 min. The membrane was washed with 1 × … Get A Quote

摘要

background: H. pylori is closely related to the occurrence and development of various digestive gastritis, peptic ulcer and mucosa-associated lymphoid tissue (MALT) lymphoma. H. pylori is also a class I carcinogen of gastric cancer. VacA is the only exocrine toxin of H. pylori, which plays a very important role in the pathogenesis of H. pylori. The production of VacA in natural circumstances is complex with heavy workload and low yield. Therefore, it is very important to obtain recombinant VacA protein which is stable and biologically active. This study therefore aims to explore the expression, purification and stable storage of VacA toxin of H. pylori in E.coli, and to provide experimental basis for further ex... More

关键词

Apoptosis, Expression, Helicobacter pylori, Preservation, Purification, VacA recombinant protein