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New Resources for the Specific and Sensitive Detection of the Emerging Tomato Brown Rugose Fruit Virus

Viruses. 2021-08; 
Joan Miquel Bernabé-Orts, Covadonga Torre, Eduardo Méndez-López, Yolanda Hernando, Miguel A Aranda
Products/Services Used Details Operation
GenParts™ DNA Fragments … A synthetic fragment containing part of the ToBRFV ORF1 was synthetized by GenScript (Piscataway, NJ, USA) and cloned in a pBluescript II KS (+) vector (Stratagene, La Jolla, CA, USA). In vitro transcription was performed with T3 polymerase (Promega, Madison, WI, USA) in … Get A Quote

摘要

Plant viruses can evolve towards new pathogenic entities that may eventually cause outbreaks and become epidemics or even pandemics. Seven years ago, tomato brown rugose fruit virus (ToBRFV) emerged, overcoming the genetic resistance that had been employed for more than sixty years against tobamoviruses in tomato. Since then, ToBRFV has spread worldwide, producing significant losses in tomato crops. While new resistances are deployed, the only means of control is the implementation of effective prevention and eradication strategies. For this purpose, in this work, we have designed, assessed, and compared an array of tests for the specific and sensitive detection of the ToBRFV in leaf samples. First, two monoclo... More

关键词

DAS-ELISA, LAMP, RT-qPCR, ToBRFV, detection, plant virus, tobamovirus