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Knockdown of miR-125b-5p inhibits the proliferation and invasion of gastric carcinoma cells by targeting RYBP

Kaohsiung J Med Sci. 2021-08; 
Fu-E Jin, Bo Xie, Hong-Zhen Xian, Ji-Hai Wang
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GenParts™ DNA Fragments … Fragments (400 bp) of the RYBP or TRIM71 3′-UTR (wild-type, wt) were custom synthesized (GenScript, Nanjing, China). Mutation of the putative miR-125b-5p target sequence within the 3′-UTR of RYBP or TRIM71 (mutated, mut) was generated using the QuikChange Site-… Get A Quote

摘要

Gastric carcinoma, one of the most aggressive and lethal human malignancies, is associated with poor prognosis despite progress in therapeutic strategies. This study examined the potential function and mechanism of action of microRNA-125b-5p (miR-125b-5p) in the pathogenesis of gastric carcinoma. We recognized that miR-125b-5p was elevated in gastric carcinoma, and its decreased expression was associated with a better prognosis. Loss-of-function assays showed that miR-125b-5p suppression inhibited the proliferative and invasive abilities of gastric cancer cells. Furthermore, RING1 and YY1-binding protein (RYBP) was found to be target gene for miR-125b-5p action; miR-125b-5p negatively regulates RYBP expression.... More

关键词

RYBP, gastric carcinoma, invasion, miR-125b-5p, proliferation