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DNA Sequencing | … coding sequence was codon optimized and synthesized by Genscript Co.,Ltd. and cloned … sonication and the supernatant was centrifuged and loaded onto Ni-NTA resin (Genscript). … This fluorogenic peptide was synthesized and purified to a > 95% purity by GenScript. The … | Get A Quote |
COVID-19 is a disease caused by SARS-CoV-2, which has led to more than 4 million deaths worldwide. As a result, there is a worldwide effort to develop specific drugs for targeting COVID-19. Papain-like protease (PLpro) is an attractive drug target because it has multiple essential functions involved in processing viral proteins, including viral genome replication and removal of post-translational ubiquitination modifications. Here, we established two assays for screening PLpro inhibitors according to protease and anti-ISGylation activities, respectively. Application of the two screening techniques to the library of clinically approved drugs led to the discovery of tanshinone IIA sulfonate sodium and chloroxine ... More